| Name: |
Theory and Application of Chromatography |
| Catalog Number: |
412012Y |
Hours/Credits: |
40/2 |
| Prerequisite(s): |
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This course is a professional basic course for master and doctor students specialized in analytical chemistry, it also can be used as a selected course for related majors postgraduate students such as biochemistry and so on. |
| Course Description: |
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Through studying the kinetics and thermodynamics of chromatographic process, the course discusses the basic theories and technologies of separation analysis. This course highlights plate theory, rate theory, thermodynamics of chromatographic retention, column technique, stationary phase, mobile phase and other general problems such as chromatographic qualitative and quantitative analysis and chromatographic preparation. In addition, the course specifically discusses the gas chromatography and liquid chromatography. It also makes an introduction about other new chromatographic techniques. The goal of this course is to make the student grasp the basic theories and techniques of chromatography and lay a solid foundation for solving related problems in practical experiments using chromatography as a separation analysis tool. |
| Course Content: |
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Chapter 1 Introduction
Basis of chromatographic separation and chromatography classification; Chromatograms and chromatographic basic parameters
Chapter 2 Chromatographic thermodynamic basis
Partition equilibrium of substance in chromatographic column; Retention characteristic and parameters of components in chromatographic column; Thermodynamic characteristic of retention value; The thermodynamic basis of dissolution and adsorption processes; The thermodynamic basis, chromatographic partition equilibrium and retention value of GC; Chromatographic retention value and molecular structure.
Chapter 3 Chromatographic kinetics
Equilibrium plate theory of chromatographic separation; Expression of chromatographic column efficiency; Factors that effect chromatographic band broadening; Separation degree and chromatographic separation equation
Chapter 4 Gas chromatography
Instrumental system of GC; Preparation of GC columns; Recommendation of GC columns and the choice of operating conditions; Chromatographic qualitative analysis and quantitative analysis; Chemical pretreatment and derivative of sample; New technologies of GC; Detection methods.
Chapter 5 Liquid chromatography
Instrumental system of LC; LC columns; Separation principal of LC and stationary phase, mobile phase; Gradient elution and separation process; Detection methods.
Chapter 6 New technologies of LC
Capillary electro chromatography (CEC); Hyphenated techniques chromatography; Microfluidic systems, etc. |
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