Chemistry
Name: Fluorescence Photoswitchable Nanoprobes and Their Roles in Super-Resolution Bioimaging
Catalog Number: 417018Y Hours/Credits: 20/1
Prerequisite(s):
Photochemistry/Photophysics, Materials chemistry
Course Description:
This course is primarily designed for postgraduate students majoring in physical chemistry, analytical chemistry, materials chemistry. This course includes introduction to the types, structural features, operation mechanisms of fluorescent nanoprobes with photoswitchable fluorescence emission properties for students, discussion of the roles that this class of nanoprobes play in super-resolution fluorescence imaging, recent advancement of far-field fluorescence microscopy techniques, spatial and temporal resolution of biological fluorescence imaging and the perspective of future development.
Course Content:
Chapter 1 Types of molecular switches and the photophysical mechanism of fluorescence switching Chapter 2 Types of photoswitchable fluorescent nanoprobes and the basic principles of constructing them. Included are single-color fluorescence On-Off switches based on Förster resonance energy transfer (FRET) mechanism, single-color fluorescence On-Off switches based on interconversion of single chromophore, dual-color fluorescence switches based on FRET mechanism, dual-color fluorescence switches based on single chromophore interconversion, and photoswitchable fluorescent protein photoswitchable fluorescent nanoprobes Chapter 3 Applications in biological detections, super-resolution cell fluorescence imaging, including in far-field fluorescence microscopy techniques, in target identification in cell fluorescence detection under the interference of autofluorescence, and in the extraction of weak target signals from complex environments
TextBooks:
Reference:
1) Z. Y. Tian, W. W. Wu, A. D. Q. Li, Photoswitchable Nanoprobes for Biological Imaging Applications, Chapter 1 in “Trace Analysis with Nanomaterials”, Wiley-VCH , 2010. 2) Z. Y. Tian, W. W. Wu, A. D. Q. Li, Photoswitchable fluorescent nanoparticles: preparations, properties and applications, ChemPhysChem. 2009, 10, 2577-2591. 3) M.Fernandez-Suarez, A. Y. Ting, Fluorescent probes for super-resolution imaging in living cells. Nat. Rev. Mol. Cell Bio. 2008, 9, 929-943. 4) M.Heilemann, P. Dedecker, J. Hofkens, M. Sauer, Photoswitches: Key molecules for subdiffraction-resolution fluorescence imaging and molecular quantification. Laser Photonics Rev. 2009, 3, 180-202.